Shipping conditions can shake up your vial. Whether the sample is liquid or powder, giving the vial a quick spin before it is opened will make sure ALL of the protein is ready when you need it.
Use the correct buffer to reconstitute
It might sound simple, but READ THE DIRECTIONS that come with the product. Some products need water for reconstitution, others need acidic solutions. The formulation of the lyophilized product in combination with the behavior of the protein dictates which reconstitution buffer is recommended for 100% solubility. With proper reconstitution buffers, you will not lose precious protein to insoluble flakes or accidentally denature it with the wrong solution conditions.
Avoid bubbles and shear stress
This the #1 issue we hear when people have trouble getting their protein to have function. DO NOT VORTEX your protein. Also, do not sonicate or pipet vigorously (to a point that there are bubbles in your sample). Proteins in solution do not like air or shear stress.
Add a carrier protein
BSA and HSA are two common carrier proteins that increase the long-term stability of recombinant proteins. It is thought that carrier proteins help to prevent proteins from sticking to the walls of the vial or to each other.
Small working aliquots
Repeated freeze-thaw cycles expose the protein sample to dramatic swings in temperature. Like any other parameter, consistency is king. Freezing your sample into smaller working aliquots ensures that the protein will only need to be thawed once before use.
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