Rat SHH

Rat SHH

Part Number: 300-40

Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates.‚There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors.

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Description

Alternate Names:  Shh, HHG-1                        Accession Number: Q63673   
Description:  Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates.  There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors.  SHH is a morphogen that is essential during vertebrate organogenesis and adult stem cell division. 
Source:  Genetically modified E.coliThis product is produced with no animal or human origin raw products.  All processing and handling employs animal free equipment and animal free protocols.
Predicted MW:  Monomer, 19.9 kDa (with 176 amino acids)
AA Sequence:  MIIGPGRGFG KRQHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKARI HCSVKAENSV AAKSDG
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS-PAGE

≥ 95%

Endotoxin

Kinetic LAL (50% confidence)

≤ 1 EUs/µg

Biological Activity

No biological activity data is available at this time

No biological activity data is available at this time


Preparation and Storage

Country of Origin:  USA         Shipping:  Room temperature              
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL    
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.   


Expiration Date                       

12 months from date of receipt when stored at -20°C to -80°C as supplied. 

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

                                                                                   

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!      

 

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Part Number: Other Terms:

Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 5 ug, Animal-Free Std: 25 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 5 ug, Std.: 25 ug, Value: 100 ug

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