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Porcine GM-CSF

Porcine GM-CSF

Part Number: 400-12

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells.‚GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells.

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Description

Accession Number: P48749
Description:  Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells.  GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells.  GM-CSF promotes immune system development and regulates neutrophil function during infection.
Source:  Genetically modified E.coli.
Predicted MW:  Monomer, 14.4 kDa (with 128 amino acids)
AA Sequence:  MAPTRSPTLV TRPSQHVDAI QEALSLLNNS NDVTAVMNKA VKVVSEVFDP EGPTCLETRL QLYKEGLQGS LTSLKNPLTM MANHYKQHCP PTPESPCATQ NINFKSFKEN LKDFLFNIPF DCWKPVKK
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA. 

Specification Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS-PAGE ≥ 95%
Endotoxin Kinetic LAL (50% confidence) ≤ 1 EUs/µg
Biological Activity TF-1 cell proliferation  ED50 ≤ 15 ng/mL


Preparation and Storage

Country of Origin:  USA
Shipping: 
Room temperature               Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.


Expiration Date                       

12 months from date of receipt when stored at -20°C to -80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!           

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

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Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 5 ug, Animal-Free Std: 20 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 5 ug, Std.: 20 ug, Value: 100 ug

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