Mouse VEGF-165

Mouse VEGF-165

Part Number: 200-34

Vascular endothelial growth factor A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. ‚ VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. ‚ VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. ‚ The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.

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Description

Alternate Names:  VEGF-A, VPF, glioma-derived endothelial cell mitogen              
Accession Number: Q00731-2
Description:  Vascular endothelial growth factor A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells.  VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways.  VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant.  The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 19.4/38.8 kDa (165/230 aa)
AA Sequence:  MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

HUVEC proliferation

≤10 ng/mL

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Part Number: Other Terms:

Additional information

Dimensions 1 × 1 × 1 cm
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Mg: 1, Midgi: 500 ug, Mini: 2 ug, Std.: 10 ug, Value: 100 ug

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