Mouse MIF

Mouse MIF

Part Number: 200-78

Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. ‚ MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression. ‚ In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor alpha (TNF-Ž±) secretion.‚ 

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Description

Alternate Names:  None               Accession Number: P34884
Description:  Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity.  MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression.  In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor alpha (TNF-α) secretion. 
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 12.5 kDa (115 aa)
AA Sequence:  MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

No biological activity data is available at this time

No biological activity data is available at this time 

 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 5 ug, Animal-Free Std: 25 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 5 ug, Std.: 25 ug, Value: 100 ug

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