Mouse M-CSF

Mouse M-CSF

Part Number: 200-08

Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells. ‚ M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. ‚ M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. ‚ Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.

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Description

Alternate Names:  MGI-IM, CSF-1              

Accession Number: P07141
Description:  Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells.  M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types.  M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy.  Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.  
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 18.2/36.4 kDa (156/312 aa)
AA Sequence:  MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

NFS-60 cell proliferation

≤10 ng/mL

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Part Number: Other Terms:

Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 2 ug, Animal-Free Std.: 10 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 2 ug, Std.: 10 ug, Value: 100 ug

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