Hematopoietic stem cells are self renewing pluripotent cells often characterized by the expression of CD34. Studies have shown that decreased expression of CD34 tends to correlate with the decreased self renewal properties and pluripotent potential. Culturing CD34+ cells in a cytokine mixture of SCF, FLT-3 Ligand, IL-6 and IL-3 for short time periods (2-7 days) allows rapid expansion of immature and more mature hematopoietic progenitor cells in cord blood or bone marrow preporations. If the goal is to only expand the most immature progenitor and stem cells, it is reccommended that a combination of TPO, SCF and FLT-3 Ligand are used (Catalog: PB-500-03). Either cytokine combination can also be supplemented with other cytokines to promote specific linages. For example, GM-CSF (Catalog 200-15) and/or G-CSF (Catalog 200-14) can be added to promote monocytes and granulocytes while EPO can be added to promote erthroid cells.
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