Description
Description: Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.
Source: Genetically modified E.coli.
Predicted MW: Dimer, 19.3/38.6 kDa (166/332 aa)
AA Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
Specification |
Method of Determination |
Acceptance Criteria |
Purity |
Reducing and Non-Reducing SDS PAGE |
≥95% |
Endotoxin |
Kinetic LAL |
≤1 EUs/µg |
Biological Activity |
HUVEC proliferation |
≤10 ng/mL |
Preparation and Storage
Country of Origin: USA
Shipping: Room temperature
Storage Prior to Reconstitution: -20°C
Product Reconstitution: Sterile water at 0.1 mg/mL
Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Expiration Date
- 12 months from date of receipt when stored at -20°C to-80°C as supplied.
- 1 month when stored at 4°C after reconstituting as directed.
- 3 months when stored at -20°C to -80°C after reconstituting as directed.
To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.
Part Number: Other Terms:
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