Accession/Uniprot Number: P40225
Description: Thrombopoietin (TPO) is a growth factor that is produced by liver and kidney tissues. TPO binds the TPO receptor (CD110) to promote megakaryocyte maturation, differentiation, and the production of platelets. Human TPO (CHO) contains an C-terminal 6x-polyhistidine (6xHis) tag.
Source: Genetically modified CHO cells.
Predicted MW: Monomer, 36.3 kDa without glycosylation (338 amino acids)
AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGHHHHHH
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 2 mM L-Arginine, pH 7.5
|Specification||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-reducing SDS-PAGE||≥90%|
|Endotoxin||Kinetic LAL (50% confidence)||≤ 1 EUs/ug|
|Biological Activity||MO7e cell proliferation||≤ 15 ng/mL|
Preparation and Storage
Country of Origin: USA
Shipping: Room temperature
Storage Prior to Reconstitution: -20°C
Reconstitution: Sterile water at 0.1 mg/mL
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
- 12 months from date of receipt when stored at -20°C to -80°C as supplied.
- 1 month when stored at 4°C after reconstituting as directed.
- 3 months when stored at -20°C to -80°C after reconstituting as directed.
THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!
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