Shenandoah Biotech Logo

Human TNF-alpha

Human TNF-alpha

Part Number: 100-111

Tumor necrosis factor alpha (TNF-Ž±)‚ is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-Ž± signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-Ž± functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.‚ 

[bulk_variations include=”4960″]

$67.00$1,471.00

Clear

Description

Alternate Names:  TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF               Accession Number: P01375
Description:  Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever. 

Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.5 kDa (158 aa)
AA Sequence:  MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Cytolysis of mouse L929 cells in the presence of Actinomycin D

≤2 ng/mL; ≥ 5.0 x 10^5 units/mg 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

Part Number: Other Terms: TNFALPHA, TNF

Additional information

Dimensions 1 × 1 × 1 cm
Select size

Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 10 ug, Animal-Free Std.: 50 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 10 ug, Std.: 50 ug, Value: 100 ug

Reviews

There are no reviews yet.

Be the first to review “Human TNF-alpha”