Description

Alternate Names:  Differentiation inhibiting factor, cartilage-inducing factor               Accession Number: P01137
Description:  Transforming growth factor-beta 1 (TGF-b1) is a member of the TGF-beta superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways. TGF-b1 signals through SMAD proteins via the TGF-bRI and TGF-bRII receptors.  TGF-b1 is often used in addition to recombinant IL-2 for production of Treg cells in cell culture.  
Source:  Genetically modified CHO cells.
Predicted MW: Dimer, 12.8/25.6 kDa (112/224 aa)
AA Sequence:  ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

 **** Reference Units: The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1 NIBSC code: 89/514.

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile 10 mM HCl at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:      

• 12 months from date of receipt when stored at -20°C to -80°C as supplied.
• 1 month when stored at 4°C after reconstituting as directed.
• 3 months when stored at -20°C to -80°C after reconstituting as directed.

Part Number: Other Terms: