T regulatory cells (Tregs) are a subset of T cells that suppress immune function. They are characterized by the expression of CD4, CD25 and Foxp3 and are known to produce immunosuppressive cytokines TGF-beta 1 and IL-10. Tregs can be cultured from naive CD4+ T cells by culturing them in the presence of IL-2 (at 50-100 U/mL) and TGF-beta 1 (at 5 ng/mL) with CD3 and CD28 stimulation. In some cases cells are also cultured with neutralizing antibodies to IL-4, IFNy and IL-12 and/or retinoic acid.
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