Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells. M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.
Source: Genetically modified E.coli.
Catalog Number: 100-03
Alternate Names: MGI-IM, CSF-1 Accession Number: P09603
Predicted MW: Dimer, 18.5/37.1 kDa (159/318 aa)
AA Sequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 8.0
M-CSF Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
|Specification||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥95%|
|Endotoxin||Kinetic LAL||≤1 EUs/µg|
|Biological Activity||NFS-60 cell proliferation||≤10 ng/mL; ≥ 1.0 x 10^5 units/mg
*** International Units: The specific activity of Human M-CSF is 1.0 x 10^5
IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).
Preparation and Storage
Country of Origin: USA
Shipping: Room temperature Storage Prior to Reconstitution: -20°C
Product Reconstitution: Sterile water at 0.1 mg/mL
Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.
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Part Number: Other Terms: MCSF