FUJIFILM Irvine Scientific

Human M-CSF

Human M-CSF

Part Number: 100-03

Human MCSF, Macrophage Colony Stimulating Factor, is a recombinant protein used in cell culture to differentiate primary PBMCs & monocytes to macrophages. Tested for biological activity in M-NFS-60 cell proliferation assay. Lyophilized protein, high purity, low endotoxin & high quality M-CSF manufactured in the USA by‚ protein manufacturer‚ Shenandoah.

[bulk_variations include=”5958″]



Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells. M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.
Source:  Genetically modified E.coli.

Catalog Number: 100-03

Alternate Names:  MGI-IM, CSF-1               Accession Number: P09603

Predicted MW: Dimer, 18.5/37.1 kDa (159/318 aa)
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 8.0

M-CSF Product Specifications*

*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥95%
Endotoxin Kinetic LAL ≤1 EUs/µg
Biological Activity NFS-60 cell proliferation ≤10 ng/mL; ≥ 1.0 x 10^5 units/mg


*** International Units: The specific activity of Human M-CSF is 1.0 x 10^5
IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date

12 months from date of receipt when stored at -20°C to-80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

Part Number: Other Terms: MCSF

Additional information

Dimensions 1 × 1 × 1 cm
Select size

Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 2 ug, Animal-Free Std.: 10 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 2 ug, Std.: 10 ug, Value: 100 ug


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