Alternate Names:  CSF-3, MGI-1G, GM-CSFβ, pluripoietin              

Accession Number: P09919
Description:  Granulocyte-colony stimulating factor (G-CSF) is a cytokine that functions as a potent inducer of neutrophilic granulocyte proliferation, terminal differentiation, and activation.  G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), or interleukin 17 (IL-17).  The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways.  G-CSF also promotes neurogenesis and inhibits neuronal apoptosis.  Human and mouse G-CSF proteins are cross-reactive. 
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 18.8 kDa (175 aa)
AA Sequence:  MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Formulation:  –  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM acetic acid, 50 mM sodium chloride.

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.