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Human FGF-basic 147 aa (FGF2)

Human FGF-basic 147 aa (FGF2)

Part Number: 100-28

Basic fibroblast growth factor (FGF-basic), also known as‚ FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. ‚ FGF-basic also has cardioprotective functions during heart injury. ‚ FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state.

 

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Description

Alternate Names:  FGF2, HBGF-2, Prostatropin               Accession Number: P09038
Description:  Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis.  FGF-basic also has cardioprotective functions during heart injury.  FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state.  Degredation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources.  The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic.  Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 16.5 kDa (147 aa)
AA Sequence:  MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mm sodium phosphate, 75 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥95%
Endotoxin Kinetic LAL ≤1 EUs/µg
Biological Activity 3T3 cell proliferation ≤5 ng/mL; ≥ 2.0 x 10^5 units/mg
Preparation and Storage:
Country of Origin:  USA        Shipping:  Room temperature
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 10 ug, Animal-Free Std.: 50 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 10 ug, Std.: 50 ug, Value: 100 ug

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