Alternate Names: FGF2, HBGF-2, Prostatropin Accession Number: P09038
Description: Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic. Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.
Source: Genetically modified E.coli.
Predicted MW: Monomer, 16.5 kDa (147 aa)
AA Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mm sodium phosphate, 75 mM sodium chloride, pH 7.5
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
|Specification||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥95%|
|Endotoxin||Kinetic LAL||≤1 EUs/µg|
|Biological Activity||3T3 cell proliferation||≤5 ng/mL; ≥ 2.0 x 10^5 units/mg|
Country of Origin: USA Shipping: Room temperature
Product Reconstitution: Sterile water at 0.1 mg/mL
Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.
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