Human BDNF

Human BDNF

Part Number: 100-01

Human BDNF,‚ Brain-derived neurotrophic factor, is a recombinant protein used as a nerve growth factor. In tissue or cell culture, it supports neuron growth and survival. BDNF is often used in combination with recombinant human GDNF for neuronal stem cell cultures.‚ Human BDNF, mouse BDNF, rat BDNF, and pig BDNF are cross-reactive.

Tested for biological activity in a C6 cell proliferation assay. Lyophilized protein, high purity, low endotoxin, high quality BDNF is manufactured in the USA by protein manufacturer‚ Shenandoah.

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[bulk_variations include=”4106″]

$67.00$5,881.00

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Description

Alternate Names:  None               Accession Number: P23560

Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Human, mouse, rat, and pig BDNF are cross-reactive.

Source:  Genetically modified E.coli.
Predicted MW: Noncovalent Homodimer, 13.6/27.3 kDa (120/240 aa)
AA Sequence:  MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*

*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

C6 cell proliferation

≤2 ug/mL; ≥ 5.0 x 10^2 units/mg

Preparation and Storage

Country of Origin:  USA   Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C 
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                   

  • 12 months from date of receipt when stored at -20°C to-80°C as supplied.
  • 1 month when stored at 4°C after reconstituting as directed.
  • 3 months when stored at -20°C to -80°C after reconstituting as directed.

 

Complementary products:
Need an Anti-Human BDNF antibody?  We recommend Anti-Human BDNF Rabbit Polyclonal Antibody from ImmunoReagents.  If you are curious to know more about the relationship between Shenandoah and Immunoreagents, click here for the scoop.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

Part Number: Other Terms: BDNF

Additional information

Dimensions 1 × 1 × 1 cm
Select size

Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 2 ug, Animal-Free Std.: 10 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 2 ug, Std.: 10 ug, Value: 100 ug

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