Description: Amphiregulin (AR) is a member of the epidermal growth factor (EGF) family. AR is an autocrine growth factor and functions to regulate cell proliferation and survival through binding the EGF receptor (EGFR). AR promotes the proliferation of neural stem cells and epithelial cells, including keratinocytes and mammary epithelium. Up-regulated at puberty, AR promotes ductal outgrowth and mammary branching. In the liver, AR functions as a mitogen and prevents hepatocyte apoptosis. In a cancer context, AR protects against human adenocarcinoma apoptosis, promotes tumor cell growth, and functions as an oncogenic factor in the Hippo pathway. AR levels are increased in the brain following stress events, such as inflammation, ischemia, and hypoxia.
Source: Genetically modified E.coli.
Predicted MW: Monomer, 10.1 kDa (with 87 amino acids)
AA Sequence: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMK
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA.
|Specification||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS-PAGE||≥ 95%|
|Endotoxin||Kinetic LAL (50% confidence)||≤ 1 EUs/µg|
|Biological Activity||3T3 Cell Proliferation||ED50 ≤ 20 ng/mL|
Preparation and Storage
Country of Origin: USA Shipping: Room temperature
Storage Prior to Reconstitution: -20°C
Product Reconstitution: Sterile water at 0.1 mg/mL
Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.
THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!
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