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Equine VEGF-165

Equine VEGF-165

Part Number: 400-18

Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells.‚VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways.‚VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant.

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Description

Alternate Names:  VEGF-A, VPF, glioma-derived endothelial cell mitogen
Accession Number: Q9GKR0
Description:  Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells.  VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways.  VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant.  The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.
Source:  Genetically modified E.coli.
Predicted MW:  Dimer, 19.3 / 38.6 kDa (with 165/330 amino acids)
AA Sequence:  MAPMAEGEHK THEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TAEFNITMQI MRIKPHQSQH IGEMSFLQHS KCECRPKKDK ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM Sodium Phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

Specification Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥95%
Endotoxin Kinetic LAL ≤1 EUs/µg
Biological Activity HUVEC Proliferation ED50 ≤ 10 ng/mL
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 5 ug, Animal-Free Std: 20 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 5 ug, Std.: 20 ug, Value: 100 ug

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