Equine PDGF-BB

Equine PDGF-BB

Part Number: 400-17

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis.‚PDGF synthesis is induced by IL-1, IL-6, TNF-Ž±, TGF-Ž², and EGF signaling.‚PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.

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Description

Alternate Names:  GDGF, ODGF                   Accession Number: XP_005606703  
Description:  Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis.  PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β, and EGF signaling.  PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.  PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues.  PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB).  The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β. 
Source:  Genetically modified E.coli
Predicted MW:  Dimer, 24.8 kDa (with 110/220 amino acids)
AA Sequence:  MSLGSLAVAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRHVQ CRPTQVQLRP VQVRKIEIVR KKPTFKKATV TLEDHLACKC ETVGAARPVT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

ED50 ≤ 30 ng/mL 


Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL 
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.   

Expiration Date                       
12 months from date of receipt when stored at -20°C to -80°C as supplied. 
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.
 

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

Part Number: Other Terms:

Additional information

Dimensions 1 × 1 × 1 cm
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Animal-Free Mg: 1 mg, Animal-Free Midgi: 500 ug, Animal-Free Mini: 5 ug, Animal-Free Std: 20 ug, Animal-Free Value: 100 ug, Mg: 1 mg, Midgi: 500 ug, Mini: 5 ug, Std.: 20 ug, Value: 100 ug

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