Shenandoah Biotech Logo

CTGrade rh IL-21

CTGrade rh IL-21

Part Number: 500-09

CTGrade interleukins and growth factors are signaling molecules that induce various cell functions or phenotypic changes and are used to stimulate and promote adaptive cellular immunity and tolerance with T cells.

CTGrade rh IL-21 induces proliferation and differentiation in target cells and synergizes with CTGrade rh IL-7 and CTGrade rh IL-15 to expand and activate CD8 T cells, as well as augment the activity of natural killer (NK) cells.

CTGrade interleukins and growth factors are designed to support basic, translational, and clinical research, as well as commercial applications.



CTGrade rh IL-21 is a human recombinant protein containing no animal-derived materials, is ≥ 97% pure, produced in E. coli, is Beta-lactam-free, and manufactured by Shenandoah in an ISO 9001:2015 certified facility that follows cGMP guidelines. CTGrade interleukins and growth factors are designed to support basic, translational, and clinical research, as well as commercial applications.*

● Extremely low endotoxin levels
● High biological activity verified by relevant bioactivity assay
● Carrier-free protein
● Available quantities: 50 µg, 100 µg, and 1 mg; custom sizes and packaging available

IL-21 is a common chain cytokine regulating many cell types of the immune system. The IL-21 receptor (IL-21R) activates the JAK/STAT signaling pathway and is expressed on T, B, and natural killer (NK) cells. IL-21 plays an important role in the development of humoral immunity through its effects on B cell biology including differentiation, affinity maturation and memory responses. IL-21 regulates T cell responses including survival of CD8+ T cells and inhibition of Treg cells. IL-21 plays a pivotal role in Hodgkin’s lymphoma and suppresses contact hypersensitivity reactions. In adoptive cellular therapies i.e., CAR T cells, IL-21 promotes a less differentiated phenotype while promoting the effector functions of the engineered cells.

Technical Specifications**
Accession Number: Q9HBE4
Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses
Predicted Molecular Mass: Monomer, 15.4 kDa (132 aa)
Formulation: 10 mM NaP, pH 7.5
Product Reconstitution: Reconstitute at 0.1 mg/mL with Water
Endotoxin level: < 0.1 EU per 1 μg of the protein by the USP 85/EP2.6.14 method
o Measured in a cell proliferation assay using a B9 cell proliferation
o The ED50 for this effect is 1.243 ng/mL
Host Cell DNA: ≤ 10 ng/mL by RT-PCR
Host Cell Protein: ≤ 30 ng/mL by ELISA
Sterility: Negative on growth by USP 71/EP2.6.1
Country of Origin: USA

Storage: Store at -20°C in a non-defrosting freezer. Transportation temperature is 2-8°C.

Shelf Life: The lyophilized product is stable for 36 months from the date of manufacture; 12 months from date of receipt when stored at -20° C to -80° C as supplied, 1 month when stored at 4°C after reconstituting as directed, and 3 months when stored at -20°C to -80°C after reconstituted as directed. Avoid repeated freeze-thaw cycles.

*For research use or further manufacturing and in ex vivo cell therapy applications.
**Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Additional information

Dimensions 1 × 1 × 1 cm
Select size

Mg: 1mg, Mini: 50 ug, Std: 100ug


There are no reviews yet.

Be the first to review “CTGrade rh IL-21”